|
KMID : 0369819910210030171
|
|
Jorunal of Korean Pharmaceutical Sciences
1991 Volume.21 No. 3 p.171 ~ p.177
|
|
|
Quantitative Analysis of Pyridostigmine Bromide in Tablets by HPLC
|
|
피택산/Phi TS
조영/석대은/차승희/정윤수/Cho Y/Sok DE/Cha SH/Chung YS
|
|
|
Abstract
|
|
|
|
A reverse-phase, ion-pair high performance liquid chromatographic (HPLC) method for the simultaneous quantative determination of pyridostigmine and its hydrolytic product, 3-hydroxy-N-methylpyridinium (HMP), is descrihed, The assay of pyridostigmine and HMP was linear in the range of amount from 24 to 60 mg/tablet and from 2.4 to 12.0 mg/tablet, respectively, with coefficient of variation (C.V.) of 0.05-0.12% (n=7) and 0.25-0.52% (n=5), respectively, and applicable conveniently even in the case of the mixture of pyridostigmine and HMP. Meanwhile, the conventional UV method gave inaccurate results for the aged pyridostigmine tablets. In the extraction of pyridostigmine from tablets prior to be assayed by HPLC, methanol was found to be more effective than ethanol or distilled water. Multiple extraction (four times) with methanol resulted in the full recovery of pyridostigmine, whereas ethanol gave 95% recovery even after four times extraction. Based on these results. the present method would be very useful for the accurate determination of pyridostigmine in the aged pyridostigmine tablets.
|
|
|
KEYWORD
|
|
|
pyridostigmine, ion-pair HPLC, pyridostigmine tablet
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|